In this October issue of Clinical Pharmacology & Therapeutics, Yang et al. highlighted several important variants in pharmacogenes that could be missed if whole exome sequencing or genotyping array was used instead of whole genome sequencing. Among the thirteen CPIC® important genes, missing important variants are found in CYP2D6, CYP3A5, DPYD, G6PD, IFNL3, VKORC1 if whole genome sequencing were not used.